Effect of some of anti frost on morphology, anatomy and proline of selective almond cultivars flower buds

In this experimental, effect of some of antifrost on morphology, anatomy content of selective almond cultivars flower buds of 3 late, medium and early flowering cultivars of almond in Pheranshahr region using factorial design base on complete block randomize with 3 replications was investigated. This region is considerate as cold temperate. Experimental trees were planted with 6×6 m in 2000. In this research, trees no irrigated. Antifrost treatments including: Thiofer, Crop aid and Fosnutren that trees were sprayed using Thiofer and Crop aid in 5 /1000 in 26 November 2010 and 19 March 2011. But Fosnutren was applied with 5 /1000 at 5 may 2011. Of course before applying of treatments, sampling from flower buds for proline determination and bud characteristics study was carried out. This work was repeated in three stage of flower bud development. Results of present study showed that proline rate was significantly decreased by development stage of flower buds in all cultivars for example proline rate in cultivars of Sanky, Azar and Shekofeh before applying of treatments was0.44, 0.52 and 0.66 micromol per fresh weight (g.). While proline rate in medium of winter in cultivars of Sanky , Azar and Shekofeh treated by Thiofer, and Crop aid was 1.25 and 0.88 , 0.1.25, and 0.82 ,0.99 and 0.90 and in end of winter 0.56 and 0.44,0.59 and 0.87 and 0.47 and 0.56 ( μmol )per fresh weight (g.) respectively. Also, it was fund that treatments no effect significantly on morphology and anatomy of selective almond cultivars flower buds.